Table 1.

Dynamics of parasite strains during culture

Genotypes of isolates			Sample	Relative ratio of genotypes
Mixed msp1	Msp2	Glurp		Day 0	Day 8	Day 14	Day 21	Day 28
K1-200:MAD20-150	FC27-300	800	N77	1:20	1:9.3×104	1:2.5×104	1:2.34×104	1:2.37×104
K1-200:MAD20-200	FC27-300	600	N36	3:1	300:1	8.5×104:1	1.78×105:1	K1
	FC27-350	700	A45	11:1	3.2×104:1	1.4×105:1	9.9×105:1	K1
	FC27-300	1000	A28	1:5	1:3.1×103	1:3.6×105	MAD20	MAD20
	FC27-400	900	A34	1:6	1:2.1×103	1:1.8×104	1:9.1×104	MAD20
	3D7-500	900	A16	1:7	1:2.6×104	1:3.5×104	1:3.47×104	1:1.59×105
	FC27-300	900	A32	1:13	1:3.3×103	1:5.4×103	1:9.55×103	1:2.82×104
	FC27-400	800	A12	1:20	1:4.2×103	1:3.6×104	1:2.29×104	1:9.54×104
	3D7-550	650	N17	1:25	1:1.9×104	1:8.3×103	1:4.17×104	MAD20
	FC27-300	900	A57	1:33	1:2.4×103	0:4.3×105	MAD20	MAD20
	FC27-300	800	A39	1:90	1:1.2×104	1:3.0×104	MAD20	MAD20
	FC27-300	900	N13	1:123	1:548	1:2.9×103	1:5.76×103	MAD20
K1-200:MAD20-250	FC27-300	800	A50	1:3	1:9.9×103	1:2.6×103	1:6.45×102	1:7.25×103
K1-250:MAD20-200	3D7-500	950	A5	2:1	1:1×104	1:1.9×104	1:7.42×104	MAD20
	FC27-350	900	A52	33:1	9.3×105:0	4.1×106:0	K1	K1

Note: Parasite msp1 genotypes have two major allele types K1 and MAD20, while msp2 genotypes have two allele types IC/3D7 and FC27. Numbers following the types indicate the sizes of the PCR products. Glurp alleles were based on the size of the PCR product only.