In vitro transfection efficiency of niosomes/DNA complexes in HeLa cells at pH 7.4 in the absence of serum (white bars) and presence of 10% serum (black bars) with weight ratios of 20, 30, and 10 for niosomes-C14, niosomes-C16, and niosomes-C18, respectively. Each value represents the mean ± S.D. of triplicate experiments