Table 1.
Sequence Analysis of 858 GFP- Positive Clones
| Category | GFP+ population | # of GFP+ clones/total | % of GFP+ clones | # of GFP+ clones that also hit a CpG island | # of GFP+ clones that also hit exon 1 or intron 1 of a Refgene |
|---|---|---|---|---|---|
| A | |||||
| Within 500 bp upstream of a Refgene transcription start site | GFP+ low | 15/418 | 4% | 9/15 | — |
| GFP+ high | 57/440 | 13% | 49/57 | — | |
| 500bp-2kb upstream of a Refgene transcription start site | GFP+ low | 8/418 | 2% | 0/8 | — |
| GFP+ high | 7/440 | 2% | 3/7 | — | |
| B | |||||
| Within 2 kb upstream of transcription start site of predicted genes in >2 annotation tables | GFP+ low | 37/418 | 9% | 13/37 | 19/37 |
| GFP+ high | 35/440 | 8% | 23/35 | 17/35 | |
| C | |||||
| CpG islands only | GFP+ low | 34/418 | 8% | 34/34 | 9/34 |
| GFP+ high | 51/440 | 12% | 51/51 | 15/51 |
Redgene refers to known genes derived from RefSeq mRNA alignments
Predicted gene annotation tables are from Genscan, Ensembl, Acembly, and Softberry