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Tissue polarity of colonic crypts. (A) A simplified schematic of colonic crypts possessing distinct proliferative (green cells) and non-proliferative (red cells) zones. (B) EGFP (green) fluorescence and immunofluorescence staining of Muc2, CGA and CA-II (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar = 100 μm.
