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ER stress increases FGF21 mRNA stability. 3T3-L1 adipocytes were pretreated with 100 nM TG/DTT or vehicle control (DMSO) for 4 h and then treated with 5.0 μg/mL actinomycin D (time 0). Total cellular RNA was extracted at 0, 0.5, 1, 2, 4, and 6 h after actinomycin D addition. FGF21 mRNA levels were determined by real-time RT-PCR. Values are mean ± S.E. of three independent experiments.
