Table 2.
Primers sequence, product length, and NCBI reference sequence for reference genes by RT-qPCR.
| Gene symbol | Name | Forward and reverse primers | Product length (bp) | NCBI reference sequence |
|---|---|---|---|---|
| ACTB | Beta-actin | 5′-CTGGAACGGTGAAGGTGACA-3′ | 140 | NM_001101.3 |
| 5′-AAGGGACTTCCTGTAACAATGCA-3′ | ||||
|
| ||||
| B2M | Beta-2-microglobulin | 5′-CTATCCAGCGTACTCCAAAG-3′ | 165 | NM_004048.2 |
| 5′-ACAAGTCTGAATGCTCCACT-3′ | ||||
|
| ||||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 5′-CTTTGTCAAGCTCATTTCCTGG-3′ | 133 | NM_002046.3 |
| 5′-TCTTCCTCTTGTGCTCTTGC-3′ | ||||
|
| ||||
| HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | 5′-AGATGGTCAAGGTCGCAAG-3′ | 128 | NM_000194.2 |
| 5′-GTATTCATTATAGTCAAGGGCATATCC-3′ | ||||
|
| ||||
| SDHA | Succinate dehydrogenase complex, subunit A, flavoprotein (Fp) | 5′-TGGTTGTCTTTGGTCGGG-3′ | 85 | NM_004168.2 |
| 5′-GCGTTTGGTTTAATTGGAGGG-3′ | ||||
|
| ||||
| YWHAZ | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | 5′-CAACACATCCTATCAGACTGGG-3′ 5′-AATGTATCAAGTTCAGCAATGGC-3′ |
133 | NM_001135699.1 |
bp: base pair. Modified from Souza et al., 2012 [17].