Table 3.
Dispersion data of raw Cq values for candidate reference genes in goiter and normal thyroid tissue.
| Tissue | Symbol gene | Mean Cq | SD | CV (%) | Minimum | Maximum | MFC |
|---|---|---|---|---|---|---|---|
| Goiter | ACTB | 21.88 | 1.78 | 8.1 | 19.78 | 25.00 | 1.26 |
| B2M | 20.83 | 2.59 | 9.3 | 18.10 | 26.11 | 1.44 | |
| GAPDH | 21.18 | 1.95 | 9.2 | 19.54 | 25.60 | 1.31 | |
| HPRT1 | 30.10 | 3.10 | 10.3 | 25.48 | 35.24 | 1.38 | |
| SDHA | 31.17 | 2.21 | 7.1 | 29.51 | 34.56 | 1.17 | |
| YWHAY | 27.48 | 2.47 | 9.0 | 22.71 | 30.93 | 1.36 | |
|
| |||||||
| Normal | ACTB | 23.56 | 1.92 | 8.1 | 19.99 | 25.69 | 1.29 |
| B2M | 24.63 | 5.08 | 20.6 | 18.53 | 32.65 | 1.76 | |
| GAPDH | 22.84 | 1.78 | 7.8 | 20.54 | 26.20 | 1.28 | |
| HPRT1 | 32.12 | 3.06 | 9.5 | 25.65 | 35.66 | 1.39 | |
| SDHA | 33.94 | 3.20 | 9.4 | 29.54 | 39.39 | 1.33 | |
| YWHAY | 26.29 | 2.35 | 8.9 | 22.83 | 29.25 | 1.28 | |
SD: standard deviation; CV: coefficient of variation; and MFC: maximum fold change (the ratio of the maximum and minimum values).