Figure 2.

Representative immunofluorescent staining of IM-therapy ((a)–(c)) and UM-therapy ((d)–(f)) ischemic hind-limb muscle, at 17 days following iliac artery ligation, CD31 staining (red); TO-PRO-3-nuclei staining (blue); colocalization of GFP and red CD31 staining (yellow). Within IM-therapy groups: (a) areas without discernable GFP signal (yellow arrows), (b) regions with strong GFP signal (white arrows), and (c) arterioles with little GFP signal (red arrows). In comparison, in UM-therapy groups, a wider diffuse of GFP signal was distributed in (d) both capillaries endothelial (yellow arrows), (e) the adjacent areas (white arrows), and (f) small- to medium-sized arterioles (red arrows). Scale bar = 50 μm. Adapted from [21].