Figure 2.

Cytotoxicity and protective effect of PTS, Rg1, Re, and R1 in PC12 cells. (a) Cytotoxicity of PTS. PC12 cells were treated with PTS at concentrations ranging from 0 to 40 μg·mL⁻¹ for 24 h. (b) Cytotoxicity of Rg1, Re, and R1. The treatment was the same as described in Panel A except with the indicated concentrations. (c) Effect of PTS on the cell survival of PC12 cells against OGD-Rep. PC12 cells were pretreated with PTS at concentrations ranging from 0 to 20 μg·mL⁻¹ or the combination of Rg1, Re, and R1 (Comb.) with each 2.5 μM for 2 h, subsequently subjected to OGD-Rep treatment for 8 h, and finally maintained in oxygenated cell culture medium for another 24 h. The cell viability was determined by CCK-8 assay. Values represent mean ± SD (n = 6). *P < 0.05 and **P < 0.01 versus OGD-Rep.