Figure 6.

High glucose reduces the activation of the transcription factors, apoptosis-inducing factor, and caspase-3 in Jurkat T cells exposed to ROT. Leukemia cells were left untreated ((a), (c), (e), (g), and (i)) or exposed to (50 μM) ROT ((b), (d), (f), (h), and (j)) in G55 medium for 24 h. Cells were stained with anti-NF-κB-p65 ((a) and (b)), anti-p53 ((c) and (d)), anti-c-Jun ((e) and (f)), anti-caspase-3 ((g) and (h)), and anti-AIF ((i) and (j)) antibodies, as described in Materials and Methods. Notice that positive nuclei (dark brown color) reflect their nuclear translocation/activation and appear to correlate with the apoptotic nuclear morphology. Magnification 500x ((a)–(j)). Insets: percentage of positive DAB staining.