Figure 2.

Diagram of derivative strains obtained in the genomic design applied to S. meliloti 1021. Original replicons are shown in color: red, pSymA; yellow, pSymB; blue, chromosome. Origins of replication are indicated as OA, OB, and OC for pSymA, pSymB, and chromosome, respectively. The sites of reiterated elements participating in cointegration are indicated: a, ISRm11; b, nodPQ genes; c, algI genes. The name of each derivative strain is indicated at the bottom of the corresponding diagram. The sites of cointegration of pSymB and the chromosome in strain CFNX602 and the site of cointegration of pSymA into the cointegrate formed by pSymB and the chromosome (represented in green) in strain CFNX611 are not known.