Table 1.
Quantification of Structures Resulting From Cointegration and Excision Events in the Cultures of Wild-Type S. meliloti 1021 and Its Derivative Strains
| Event | Sitea | Primersa | Control strain | Test strain | nb | Fc |
|---|---|---|---|---|---|---|
| Cointegration of pSymA,pSymB | a | 1-6 | CFNX601 | wild type | 12 | 2.4 × 10-4 |
| a | 2-5 | CFNX601 | wild type | 13 | 1.2 × 10-4 | |
| b | 3-8 | CFNX603 | wild type | 12 | 2.4 × 10-4 | |
| b | 4-7 | CFNX603 | wild type | 13 | 1.2 × 10-4 | |
| Excision from CFNX601 | a | 1-2 | wild type | CFNX601 | 10 | 1 × 10-3 |
| a | 5-6 | wild type | CFNX601 | 10 | 1 × 10-3 | |
| b | 3-8 | CFNX603 | CFNX601 | 12 | 2.4 × 10-4 | |
| b | 4-7 | CFNX603 | CFNX601 | 13 | 1.2 × 10-4 | |
| Excision from CFNX603 | a | 1-6 | CFNX601 | CFNX603 | 12 | 2.4 × 10-4 |
| a | 2-5 | CFNX601 | CFNX603 | 12 | 2.4 × 10-4 | |
| b | 3-4 | wild type | CFNX603 | 10 | 1 × 10-3 | |
| b | 7-8 | wild type | CFNX603 | 10 | 1 × 10-3 |
a
Site of event and position of the primers are shown schematically in Fig. 4. The sequence and location of the primers are given in Methods
b
“n” represents the difference in the number of cycles necessary to obtain the same amount of PCR product between the test and the control (see Methods). n values are the mean of four independent measurements
c
“F” represents the relative concentration of rearranged structures in the culture (see Methods)