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. 2014 Mar 14;99(6):E1137–E1145. doi: 10.1210/jc.2013-4161

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Copyright © 2014 by the Endocrine Society

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Figure 5. — Effect of vit D3 on AMH-induced phospho-Smad 1/5/8 nuclear localization. Immunofluorescence (a, b, and c) and phase contrast (d, e, and f) micrographs of human GCs (n = 4) cultured with media alone (a), recombinant AMH (b), or recombinant AMH with vit D3 (c) is shown. The positive signal is seen in green. Recombinant AMH markedly increased nuclear staining when compared with the controls. When GCs were treated with vit D3 in the presence of recombinant AMH, nuclear staining was significantly reduced. Bars represent mean ± SEM. *, P < .0001 between control vs AMH; **, P < .0001 between control vs AMH + vit D3 treated and between AMH vs AMH + vit D3 treated.