Table 2.

Summary of In Situ Hybridization Results

Expression	Correlation	Probability	UniGene ID	Annotation	IMAGE clone	In situ hybridization result
105	1.00	0.99	Mm.3153	Smooth muscle myosin heavy chain	1547017	SMC marker
91	0.76	0.77	Mm.31259	SM-MAO	1166836	SMC marker
344	0.66	0.42	Mm.16537	Alpha smooth muscle actin	1138966	SMC marker
284	0.57	0.15	Mm.205997	LPP	2698658	SMC marker
307	0.56	0.14	Mm.36850	Smoothelin	1162749	SMC marker
147	0.56	0.14	Mm.7342	PDZ and LIM domain 3	332852	SMC marker
29	0.56	0.13	Mm.212349	Myopodin	1617930	SMC marker
253	0.52	0.08	Mm.36769	SLAP	1025661	SMC marker
216	0.43	0.02	Mm.12842	Vinculin	1096898	SMC marker
134	0.57	0.16	Mm.28649	Putative serine protease	5057524	SMC marker-related
76	0.56	0.13	Mm.119265	No annotation	347893	SMC marker-related
94	0.54	0.11	Mm.1237	Pmp22	1121305	SMC marker-related
118	0.53	0.09	Mm.27390	Similar to human myoneurin	1195532	SMC marker-related
58	0.52	0.08	Mm.31552	No annotation	1365251	SMC marker-related
9	0.75	0.75	Mm.156846	Putative myosin ATPase	1137251	Nonselective
10	0.65	0.40	Mm.160140	No annotation	3412898	Nonselective
76	0.62	0.27	Mm.17807	No annotation	1532956	Nonselective
6	0.58	0.18	Mm.31071	No annotation	1052804	Nonselective
34	0.57	0.15	Mm.34461	Putative ATPase	643914	Nonselective
51	0.56	0.13	Mm.314	No annotation	1529589	Nonselective
66	0.54	0.10	Mm.183030	Weakly similar to ankyrin	1494583	Nonselective
77	0.50	0.06	Mm.41387	No annotation	337790	Nonselective
229	0.48	0.04	Mm.28406	unc93 homolog B 86% similarity	1245118	Nonselective
352	0.48	0.04	Mm.184314	Putative dioxygenase	479719	Nonselective
69	0.48	0.04	Mm.45173	Msr2	390123	Nonselective
101	0.47	0.04	Mm.22588	Similarity to human supervillin	949239	Nonselective
214	0.45	0.03	Mm.10211	Entpd5	579841	Nonselective
117	0.41	0.02	Mm.32011	Kelch-related	458907	Nonselective
349	0.38	0.01	Mm.34315	Similar to synembryn	5055713	Nonselective
410	0.37	0.01	Mm.28793	Snap25bp	465348	Nonselective
31	0.65	0.40	Mm.57221	Hoxb13, homeobox B13	1026377	Technical failure
222	0.58	0.19	Mm.34060	No annotation	679316	Technical failure
55	0.58	0.17	Mm.45019	No annotation	2938912	Technical failure
7	0.58	0.17	Mm.139005	No annotation	2780347	Technical failure
189	0.57	0.16	Mm.38877	No annotation	4000106	Technical failure
60	0.57	0.15	Mm.23983	Niban protein	1344864	Technical failure
139	0.56	0.15	Mm.22941	Putative methyltransferase	1449933	Technical failure
7	0.51	0.07	Mm.173134	No annotation	577206	Technical failure
100	0.49	0.05	Mm.196527	Ankyring repeat domain protein	1514780	Technical failure
26	0.48	0.04	Mm.33974	Unc gene homolog	1617070	Technical failure
121	0.44	0.02	Mm.30693	Basic Krüppel-like factor 4	3464873	Technical failure
181	0.43	0.02	Mm.25707	No annotation	1066724	Technical failure
245	0.42	0.02	Mm.29865	Zinc finger domain-containing	425825	Technical failure
80	0.41	0.02	Mm.139238	No annotation	402913	Technical failure
211	0.40	0.01	Mm.28119	Sgpl1	1531669	Technical failure
245	0.37	0.01	Mm.31353	Vps16 gene product	4317642	Technical failure

In situ hybridization validation of predicted SMC-specific genes. Forty-six EST clusters were chosen for validation by in situ hybridization in mouse E17.5 embryos. Genes were classified as SMC markers, SMC marker-related, nonselective, and technical failure, according to criteria described in Results. Expression, Number of detections in UniGene; correlation, Pearson's correlation with SM-MHC in the raw data set (see Methods); probability, logistic regression probability for a gene to be an SMC marker; IMAGE clone, probe template clone ID (see Methods). These results are visualized in Fig. 8. The logistic regression estimate of the total number of SMC markers in the list shown is 7.4; standard error 2.1