Table 3.

Prediction of Specific Expression in Other Tissues

Expression	Correlation	ID	Annotation
A. Prediction of cardiac-specific genes
270	1.00	Mm.3776	Myosin heavy chain, cardiac muscle, adult
127	0.82	Mm.155714	Myosin, heavy polypeptide 7, cardiac muscle, beta
59	0.82	Mm.10728	Myosin binding protein C, cardiac
389	0.82	Mm.686	Actin, alpha, cardiac
137	0.72	Mm.1529	Myosin light chain, phosphorylatable, cardiac ventricles
58	0.71	Mm.20271	Fibrillin 2
47	0.69	Mm.19961	ESTs, highly similar to atrial natriuretic factor
201	0.67	Mm.632	Troponin T2, cardiac
51	0.66	Mm.46514	Myosin light chain, regulatory A
11	0.66	Mm.89854	A disintegrin and metalloprotease domain 11
6	0.66	Mm.171448	ESTs, weakly similar to mouse forkhead protein O1A
10	0.64	Mm.207070	Mus musculus heart alpha-kinase mRNA, partial cds
43	0.64	Mm.89727	CD8beta opposite strand
5	0.60	Mm.33561	ESTs
5	0.60	Mm.125583	ESTs
5	0.60	Mm.208235	ESTs
6	0.59	Mm.158756	RIKEN cDNA 2610019F11 gene
8	0.59	Mm.27037	ESTs
52	0.59	Mm.17235	Cysteine-rich protein 3
109	0.58	Mm.43	Myosin light chain, alkali, cardiac atria
136	0.57	Mm.6375	Glycogenin 1
7	0.57	Mm.89976	Zinc finger, imprinted 1
11	0.57	Mm.210460	ESTs
5	0.56	Mm.209027	ESTs
12	0.56	Mm.218070	ESTs
36	0.56	Mm.4211	Solute carrier family 8 (sodium/calcium exchanger), member 1
7	0.56	Mm.41974	Nkx2.5/tinman homolog
8	0.55	Mm.116789	Repressor of GATA
5	0.55	Mm.160735	ESTs
5	0.55	Mm.189024	ESTs
B. Prediction of skeletal muscle-specific genes
341	1.00	Mm.1000	Myosin light chain, alkali, fast skeletal muscle
31	0.80	Mm.57093	Calcium channel, voltage-dependent, gamma subunit 1
18	0.77	Mm.18125	Expressed sequence AI118095
28	0.74	Mm.33171	Ankyrin repeat and SOCs box-containing protein 5
51	0.74	Mm.140604	RIKEN cDNA 1110002H13 gene
428	0.71	Mm.214950	Actin, alpha 1, skeletal muscle
124	0.71	Mm.29358	RIKEN cDNA 2700055K07 gene
156	0.71	Mm.29994	Enolase 3, beta muscle
260	0.70	Mm.14526	Myosin light chain, phosphorylatable, fast skeletal muscle
121	0.69	Mm.14546	Troponin T3, skeletal, fast
107	0.69	Mm.20829	Epithelial membrane protein 3
254	0.68	Mm.39469	Troponin I, skeletal, fast 2
43	0.68	Mm.46232	Integrin beta 1 binding protein 2
38	0.67	Mm.4583	Cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle)
41	0.67	Mm.144259	RIKEN cDNA 2310050C09 gene
52	0.65	Mm.31099	ADP-ribosyltransferase 1
152	0.65	Mm.36900	Troponin I, skeletal, slow 1
135	0.65	Mm.1583	Lymphocyte antigen 6 complex, locus C
12	0.65	Mm.11	MRF4
14	0.64	Mm.58214	RIKEN cDNA 2310002L13 gene
78	0.64	Mm.712	Troponin C, cardiac/slow skeletal
22	0.64	Mm.140151	Small proline-rich protein 1B
51	0.63	Mm.125614	Myosin binding protein H
21	0.63	Mm.2810	Cholinergic receptor, nicotinic, gamma polypeptide
27	0.63	Mm.45734	RIKEN cDNA 2310024D23 gene
110	0.63	Mm.878	Lymphocyte antigen 6 complex, locus D
24	0.63	Mm.45137	RIKEN cDNA 2310032K21 gene
34	0.62	Mm.10194	Myomesin 2
272	0.62	Mm.43831	Lectin, galactose binding, soluble 1
19	0.61	Mm.36668	RIKEN cDNA 1110008K04 gene
C. Prediction of retina-specific genes
67	1.0	Mm.8008	Cone-rod homeobox containing gene
92	1.0	Mm.1205	Rod photoreceptor 1
94	1.0	Mm.1372	Phosphodiesterase 6B, cGMP, rod receptor, beta
37	1.0	Mm.78749	Retinitis pigmentosa 1 homolog (human)
418	1.0	Mm.69061	Alpha transducin
23	1.0	Mm.206228	ESTs
62	1.0	Mm.59151	ESTs
830	1.0	Mm.2965	Similar to rhodopsin
85	1.0	Mm.151562	Similar to retinol-binding protein
6	1.0	Mm.172488	ESTs
38	1.0	Mm.194050	ESTs
31	1.0	Mm.1370	Phosphodiesterase 6A, cGMP-specific, rod, alpha
15	1.0	Mm.23793	Cyclic nucleotide gated channel, cGMP gated
38	1.0	Mm.42102	Tubby like protein 1
38	1.0	Mm.41982	Retinoschisis 1 homolog (human)
23	1.0	Mm.39200	Phosphodiesterase 6G, cGMP-specific, rod, gamma
114	1.0	Mm.1276	Retinal S-antigen
29	1.0	Mm.95707	RIKEN cDNA A930007I01 gene
82	1.0	Mm.679	Rod outer segment membrane protein 1
59	1.0	Mm.20422	Neural retina leucine zipper gene
D. Prediction of lens-specific genes
135	1.0	Mm.1228	Crystallin, alpha A
25	0.9	Mm.30374	Crystalline, gamma C
7	0.9	Mm.130559	ESTs
16	0.9	Mm.127184	ESTs, weakly similar to A39757 beta-crystallin
64	0.9	Mm.22830	Crystalline, beta A1
9	0.9	Mm.22861	ESTs
5	0.9	Mm.215166	ESTs
7	0.9	Mm.209940	ESTs
45	0.9	Mm.89477	Crystalline, gamma F
48	0.9	Mm.31625	Major intrinsic protein of eye lens fiber
13	0.9	Mm.95578	ESTs
17	0.9	Mm.86656	Crystalline, beta A2
22	0.9	Mm.168942	BR3B
7	0.9	Mm.180528	ESTs
25	0.9	Mm.138345	ESTs
93	0.9	Mm.127171	Beaded filament structural protein in lens-CP94
5	0.9	Mm.209953	ESTs
26	0.9	Mm.215250	Solute carrier family 7
9	0.9	Mm.137178	RIKEN cDNA E130202I16 gene
11	0.9	Mm.138333	Ras p21 GTPase

Nearest neighbor searches were performed with four different tissue-specific genes: cardiac-specific myosin heavy chain (A), skeletal muscle myosin light chain (B), the retina-specific cone-rod homeobox (C), and the lens-specific genes crystallin alpha A (D). Top 30 genes are shown in A and B. Top 20 genes are shown in C and D. On the basis of literature validation, genes specifically expressed in each tissue were identified (underlined in each list). Expression, Number of detections in UniGene