Figure 4.

Effects of ionising radiation, cisplatin or Rac1 inhibitor on cytoplasmic and nuclear Rac1 expression in HNSCC cells. Western blotting analysis of Rac1 expression in cytoplasmic and nuclear fractions obtained from parental and treatment-resistant IRR HNSCC cells before and at 24 h after treatment with ionising radiation (2 Gy), cisplatin (10 μM) or Rac1 inhibitor (20 μM). IDV ratio was calculated as ratio between IDV values of treated cells to those in untreated HNSCC. For IDV evaluation, the background corrections have been done for all protein band densities using α-tubulin for cytoplasmic and histone H3 for nuclear fractions.