Table 1.

Flow-induced fold changes in gene transcripts revealed by microarray analysis.

Gene	GO Biological Process	0 hours	2 hours	8 hours	24 hours
Cxcl1	Inflammatory response	2.59*	2.88**	1.58	−1.05
Cxcl2	Chemotaxis	2.54	4.33*	1.76	1.05
Cxcl5	Cytokine production	2.22	3.48**	1.73	1.06
Zc3h12a	Angiogenesis	1.53	2.31**	1.22	1.06
Pdlim5	Regulation of synaptogenesis	1.52**	1.05	−1.03	−1.08
Ereg	Angiogenesis	1.37	1.72**	1.17	1.04
Ripk2	Positive regulation of cytokine-mediated signaling pathway	1.26	1.54*	1.07	−1.05
Csf1	Ossification	1.19	1.19*	1.05	−1.01
Nfkb1	Negative regulation of cytokine production	1.17	1.23*	1.07	1.03
Creb1	cAMP responsive element binding protein 1	1.09	1.00	1.21*	1.00
Pik3r1	Positive regulation of protein phosphorylation	−1.00	1.51*	1.11	−1.05
Nr2f2	Negative regulation of transcription	−1.07	−1.54*	−1.18	−1.09
Ndrg1	Mast cell activation	−1.14	−1.53**	−1.02	1.03
Afp	Ovulation from ovarian follicle	−1.14	1.45	1.97**	1.01
Osr1	Metanephros development	−1.16	−1.51*	−1.21	−1.04
Pgm2	Carbohydrate metabolic process	−1.22	−1.52**	−1.05	−1.04
Pias1	Negative regulation of transcription	−1.23	−1.24*	−1.01	−1.12
Slc2a1	Transport	−1.27	−1.62**	1.08	1.01
Sap30	Negative regulation of transcription	−1.32	−1.51*	1.06	−1.12
Scd2	Lipid metabolic process	−1.40	−1.64*	−1.20	−1.07
Ero1l	Protein folding	−1.41	−1.67*	−1.06	−1.01
Egln1	Response to hypoxia	−1.48	−1.90**	−1.11	−1.03
Selenbp1	Transport	−1.59	−2.37**	−1.71	1.06
Higd1a	Response to stress	−1.91**	−2.08**	1.06	−1.06
Bnip3	Response to hypoxia	−2.52	−3.96*	−1.12	1.03
Ccng2	Cell cycle	−4.62**	−2.84	1.03	−1.04

Flow vs. non-flow fold increase or decrease for all gene transcripts having at least ±1.5-fold change at one or more time points (exceptions are Nfkb1, Csf1, Creb1, and Pias1) at a significance of p<0.1 (*) or p<0.05 (**). Results are from microarray analysis of MLO-Y4 cells exposed to 2 hours OFF and post-incubated for the given time.