Table 3.

Flow-induced fold changes in protein levels inferred from liquid chromatography-mass spectrometry.

Protein	GO Biological Process	0 hours	2 hours	8 hours	24 hours
Hnrnpu	mRNA Processing	1.25	1.09	1.26*	−1.06
FUSE-binding protein 2	mRNA Processing	−1.03	−1.04	−1.03	−1.48*
Cavin-1	Transcription	−1.03	1.01	−1.35*	1.28
HMG-I(y)	Transcription	−1.31	−1.14	1.05	−1.43*
Efemp1	Transcription	−1.08	1.05	−1.27*	1.08
Dpy-30L	Transcription	−1.32	−1.07	−1.25	−2.23*
Psmc4	Protein catabolism, folding, and transport	−1.34	−1.34	−1.40*	−1.08
Tcp-1 zeta	Protein catabolism, folding, and transport	1.07	1.11	−1.36*	1.00
Rab GDI beta	Protein catabolism, folding, and transport	−1.05	−1.05	−1.31*	−1.05
Nucleoside diphosphate kinase B	Biosynthetic processes	1.36	1.22	1.97*	−1.15
C1-THF synthase	Biosynthetic processes	−1.07	−1.04	−1.76*	−1.13
Valyl tRNA synthase	tRNA aminoacylation	−2.07	1.14	−1.39*	−1.17
Nucleolin	Nucleotide binding	−2.48	−1.24	−1.69*	1.27
CCN family member 2	Regulation of cell growth	1.59	1.33*	1.43	1.10
MCG11809	Translation	−1.04	1.01	−1.25*	−1.02
Putative uncharacterized protein	DNA replication	−1.20	1.27*	−1.18	1.18
Hemoglobin alpha adult chain	Heme, oxygen binding	−1.14	−1.27	1.55*	−1.22
S100-A6 (Calcyclin)	Not indicated	−1.11	−1.14	2.43*	−1.75*
Deoxyuridine triphosphatase	Not indicated	−1.23	1.04	−1.51*	1.13
Fibronectin	Not indicated	−1.02	−1.08	−1.40*	−1.27
similar to Tubulin, alpha 3C isoform 3	Not indicated	1.20	1.11	1.48*	−1.06
G protein-coupled receptor kinase 6	Not indicated	1.19	−1.18	1.51*	−1.04
similar to ribosomal protein L39	Not indicated	−1.09	1.04	−1.28*	1.34
ribosomal protein SA	Not indicated	1.03	1.30*	−1.02	1.16

Flow vs. non-flow fold increase or decrease in MLO-Y4 protein levels inferred from peptides detected by liquid chromatography-mass spectrometry. Proteins listed with associated gene ontology biological process and fold-change at each post-incubation time point. n=3, (*) indicates p<0.05. Proteins of special interest (discussed further in text) are underlined.