Table 2.
PMSG Mass Predictions Generated From the DNA Sequencing Results Showing Two Possible Bases at Four Positions in the DNA Being Analyzed
|
Base present at SNP locations
|
Predicted masses
|
|||||
|---|---|---|---|---|---|---|
| 1147 | 1166 | 1250 | 1291 | Forward | Reverse | Allele |
| G | A | G | C | 12311.5 | 12137.3 | I |
| G | A | G | T | 12311.5 | 12209.3 | IV |
| G | A | A | C | 12369.5 | 12127.2 | |
| G | A | A | T | 12369.5 | 12199.3 | |
| G | G | G | C | 12339.5 | 12103.2 | |
| G | G | G | T | 12339.5 | 12175.3 | |
| G | G | A | C | 12397.5 | 12093.2 | |
| G | G | A | T | 12397.5 | 12165.3 | |
| C | A | G | C | 12310.5 | 12236.4 | |
| C | A | G | T | 12310.5 | 12308.5 | |
| C | A | A | C | 12368.5 | 12226.4 | II |
| C | A | A | T | 12368.5 | 12298.4 | |
| C | G | G | C | 12338.5 | 12202.4 | |
| C | G | G | T | 12338.5 | 12274.4 | |
| C | G | A | C | 12396.5 | 12192.3 | III |
| C | G | A | T | 12396.5 | 12264.4 | |
The masses are shown for the natural forward reading frame and the reverse reading frame used for PMSG analysis of exon 3. The final column shows the alleles that were identified in this study