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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: JAMA Otolaryngol Head Neck Surg. 2013 Sep;139(9):937–943. doi: 10.1001/jamaoto.2013.4104

Fig. 1.

Fig. 1

Effects of mutations in PspA, and Ply on bacterial growth. a) At 48 hours after inoculation, CFUs in middle ear effusions were significantly lower (P < 0.001) for the Ply− and PspA− mutants compared to the wild-type (Wt) and Ply−/PspA− strains. Significant differences (P < 0.001) in CFU counts were seen between Ply− and PspA− mutants. Counts for the Wt and the Ply−/PspA− strains were highly increased while for the Ply− remained near the initial inoculum levels. There were no detectable CFU levels in the PspA− group. b) In middle ear effusions, CFUs of the PspA− mutant decreased steadily overtime with only a few remaining at 30 hours after inoculation. After 43 hours in Todd Hewitt broth (THB), however, there was an increase of about 2 logs.