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Immunofluorescence image from AD post-mortem tissue with Hoechst-labeled nuclei, FITC-tyramide-labeled gephyrin (antibody 1), and cy3-labeled β-amyloid with overlay image combining the 3 images (blue-Hoechst, green-gephyrin, red-β-amyloid). Grayscale images are shown for individual channels to improve visualization of all areas of immunoreactivity, including those areas with less intense staining. Scale bar: 10 µm.
