Immunofluorescence image from AD post-mortem tissue with Hoechst-labeled nuclei, FITC-tyramide-labeled gephyrin (antibody 1), and cy3-labeled tau with overlay image combining the 3 images (blue-Hoechst, green-gephyrin, red-tau/paired helical filament (PHF)). Grayscale images are shown for individual channels to improve visualization of all areas of immunoreactivity, including those areas with less intense staining. White arrows indicate gephyrin and tau colocalization. Scale bar is 10 µm.