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Effects of 4-HNE on DU145 and LNCaP cell apoptosis analyzed by flow cytometry with Annexin V-FITC, PI staining, and TUNEL assay. Annexin V-FITC in conjunction with PI staining was used to distinguish early apoptotic from late apoptotic or necrotic cells. Fluorescence intensity for Annexin V-FITC is plotted on the x-axis, and PI is plotted on the y-axis
