Fig. 3.

Effect of 4-HNE on phosphorylation of JNK in LNCaP (A) and DU145 cells (B): LNCaP and DU145 cells were treated with different concentrations of 4-HNE (0–40 μM) for 24 h at 37°C. The cells were scraped, collected and then washed with ice cold PBS, and the cell lysates were prepared as described in the Materials and methods section. The cell extracts (50 μg of protein) were subjected to Western blot analyses using anti-JNK and anti-pJNK antibodies. GAPDH was used as a loading control