Table 3. Dominant TCR sequences identified by massively parallel sequencing after stimulation with WT or mutant peptides.

Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.

Vβ		CDR3		Jβ	CDR3 length (amino acids)	Total no. of sequences	No. of unique sequences
SCL-42 WT-stimulated TCRs
Vβ7	LCASS	PNELGQGSAF	FGQGT	Jβ1.1	10*	160	1
Vβ12	FCASS	DEVNTEAF	FGQGT	Jβ1.1	8*	18	1
Vβ24	LCATS	TGTVMNTEAF	FGQGT	Jβ1.1	10	1332	6
SCL-42 mutant-stimulated TCRs
Vβ7	LCASS	ESWTDYGYT	FGSGT	Jβ1.2	9*	97	1
Vβ12	FCASS	DGGTRHEQF	FGPGT	Jβ2.1	9*	160	2
Vβ24	LCATS	RDTVNQPQH	FGDGT	Jβ1.5	9*	2066	17

^*Corresponds to the expected length based on TCR spectratyping.