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. 2014 May 29;3:5. doi: 10.1186/2046-2530-3-5

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Copyright © 2014 Vadnais et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantitative RT-PCR of Mfn2 (A) and Mns1 (B) in somatic and reproductive tissues. The expression level was determined as the fold-increase over baseline in mouse skeletal muscle. Gapdh and ribosomal protein S16 were used as expression control genes. Mfn2 and Mns1 were highly expressed in spermatogenic cells, decreasing from pachytene spermatocytes to condensing spermatids. Br: brain, Hr: heart, Ln: lung, Tr: trachea, Kd: kidney, Lv: liver, Sp: spleen, Sk: skeletal muscle, T: testis, Ov: ovary, Od: oviduct, Ut: uterus, PS: pachytene spermatocytes, RS: round spermatids, CS: condensing spermatids. Numbers on the ordinate represent fold-increase over baseline (mouse skeletal muscle).