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. 2014 May 29;3:5. doi: 10.1186/2046-2530-3-5

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Copyright © 2014 Vadnais et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunoblot of somatic and reproductive tissues probed with antibodies against MFN2 (top) or MNS1 (bottom). MFN2 was seen in brain, heart, kidney, and liver (top, left panel) and was found in all reproductive tissues examined (top, right panel). MNS1 was seen faintly in the kidney (bottom, left panel) and was present as two closely migrating bands in all reproductive tissues and isolated cells examined except pachytene spermatocytes, condensing spermatids, and uterus (bottom, right panel). Br: brain, Hr: heart, Ln: lung, Tr: trachea, Kd: kidney, Lv: liver, Sp: spleen, Sk: skeletal muscle, PS: pachytene spermatocytes, RS: round spermatids, CS: condensing spermatids, Cp: caput epididymal sperm, Cd: epididymal sperm, T: testis, Ov: ovary, Od: oviduct, Ut: uterus. Numbers to the left of the panels indicate the molecular weights of standard proteins (×10^-3). Asterisk (*) indicates expected size (MFN2: M_r of 80 and MNS1: M_r of 60) of protein.