A. Cells were infected with empty vector, or a retroviral construct encoding KIF12. The mRNA expression levels were determined by RT-PCR, using KIF12-specific primers. GAPDH was used as a control. B. The protein level was determined by Western analysis, using total cell lysates. Actin was used as a control. C. Cell survival assays were performed in cells infected with empty vector or with a retrovirus encoding KIF12, followed by treatment with docetaxel. The fraction of surviving cells was determined by normalizing the data from docetaxel-treated cells to untreated controls. Data shown represent means ± SD of three experiments, in which each measurement was performed in triplicate. **, P < 0.005. Similar results were obtained for KIFC3 and KIF5A as previously reported [12].