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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Immunol. 2014 Apr 23;192(11):5130–5139. doi: 10.4049/jimmunol.1301677

Figure 6. Calpain-1 deficiency leads to decreased IgE-dependent IκBα phosphorylation but not MAPK, Akt, or GSK3β.

Figure 6

Wild-type (+/+) and calpain-1 null (−/−) BMMCs were sensitized with anti-TNP IgE and stimulated with TNP-BSA for various times. Total cell lysates were analyzed by Western blotting for various phosphorylated and total proteins. A–B, Decrease IκB phosphorylation in calpain-1 null BMMCs was found at 5 min after TNP stimulation when compared with that in wild-type cells. Data are mean ± SEM. n = 3 cultures of BMMCs, **p< 0.01 by comparison with calpain-1 null BMMCs. C, The pattern of TNP-induced phosphorylation of Erk1/2, p38, JNK, Akt, and GSK3β is similar between wild-type and calpain-1 null BMMCs.