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Simvastatin inhibits binding of PC3 cells to the endothelial monolayer. (A–D) PC3 cells expressing GFP were treated with 25 µM simvastatin for 4 h (A and B) and 12 h (C and D), and introduced into 12-well plates containing a confluent monolayer of HMVECs. One hour later, medium was removed, wells were washed with PBS and the number of GFP-positive PC3 cells were determined.
