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. 2014 May 15;13:108. doi: 10.1186/1476-4598-13-108

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Copyright © 2014 Amand et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DUSP3 downregulation affects HUVEC spheroids sprouting. (Ai) Western blot and quantification of DUSP3 protein expression in siCTL, siDUSP3-1 and siDUSP3-2 transfection conditions represented as a ratio of DUSP3 on GAPDH. (Aii) Representative images of spheroid sprouting assay performed with endothelial cells as indicated with siCTL, siDUSP3-1 and siDUSP3-2 in the presence of PMA (75 ng/mL) or b-FGF (10 ng/mL). (Aiii) The mean cumulative number of sprouts per spheroid was assessed after 48 hours. Results are presented as mean ± SEM. *, P < 0,5; **, P < 0,01.