Table 1.
Summary of Amplicons Screened and Mutations Found
|
Gene
|
Amplicon
|
Screening
|
No. of mutations
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Name | Cds (bp) | Name | Length/cdsc(bp) | Success (%) | Screened (Mb) | Total | Recovered | Nonsense | Splice | Missense | Silent | Noncoding | Mutation frequency (bp/mutation) |
| gene1 | 690 | A | 569/264 | 88 | 2.3 | 16 | 6 | — | — | — | 1 | 5 | 384,553 |
| gene2 | 1434 | A | 777/381 | 74 | 2.6 | 28 | 15 | — | 1 | 6 | 2 | 6 | 176,634 |
| gene3 | 1821 | A | 639/327 | 80 | 2.4 | 22 | 18 | 1 | 2 | 7 | 3 | 5 | 130,867 |
| gene4 | 4860a | A | 911/879 | 81 | 3.4 | 21 | 14 | 1 | — | 8 | 5 | — | 242,878 |
| gene5 | 1239 | A | 812/327 | 76 | 2.8 | 24 | 18 | 2 | 1 | 2 | 3 | 10 | 157,983 |
| gene6 | 951 | A | 541/436 | 33d | 0.8 | 6 | 6 | 1 | — | 2 | 3 | — | 137,111 |
| gene7 | 2271b | A | 590/264 | 68 | 1.8 | 24 | 6 | — | 1 | 2 | 1 | 2 | 308,122 |
| gene8 | 3240b | A | 442/270 | 79 | 1.6 | 14 | 6 | — | 1 | 2 | 2 | 1 | 268,170 |
| gene9 | 1122 | A | 841/279 | 78 | 3.0 | 34 | 20 | — | — | 7 | 2 | 11 | 151,138 |
| B | 448/230 | 81 | 1.7 | 9 | 7 | — | 1 | 2 | 2 | 2 | 238,879 | ||
| gene10 | 1410 | B | 664/395 | 64 | 2.0 | 8 | 4 | 1 | — | 3 | — | — | 489,554 |
| gene11 | 4110 | A | 720/720 | 87 | 2.9 | 27 | 11 | — | — | 6 | 5 | — | 262,405 |
| gene12 | 480 | A | 496/469 | 87 | 2.0 | 13 | 6 | — | — | 3 | 3 | — | 331,407 |
| gene13 | 2151 | A | 816/443 | 75 | 2.8 | 17 | 9 | 1 | — | 4 | 1 | 3 | 313,344 |
| gene14 | 8532b | A | 953/953 | 83 | 3.6 | 28 | 16 | — | — | 14 | 2 | — | 227,805 |
| B | 873/873 | 80 | 3.2 | 22 | 13 | 1 | — | 7 | 5 | — | 247,556 | ||
| C | 813/813 | 91 | 3.4 | 20 | 10 | — | — | 5 | 5 | — | 340,914 | ||
| G | 921/881 | 87 | 3.7 | 22 | 11 | 1 | — | 6 | 4 | — | 335,659 | ||
| gene15 | 6912b | A | 955/955 | 89 | 4.4 | 25 | 18 | 2 | — | 12 | 4 | — | 244,480 |
| gene16 | 6524 | A | 798/775 | 90 | 3.3 | 26 | 17 | 2 | — | 9 | 6 | — | 194,674 |
| C | 752/418 | 93 | 3.2 | 32 | 16 | 1 | — | 8 | 2 | 5 | 201,416 | ||
| E | 736/526 | 81 | 2.7 | 20 | 8 | — | — | 4 | 2 | 2 | 343,388 | ||
| Average | 2984 | 730/540 | 79 | 2.7 | 20.8 | 11.6 | 0.6 | 0.3 | 5.4 | 2.9 | 2.4 | 234,625 | |
| Total | 47,747 | 16,067/11,878 | 59.8 | 458 | 255 | 14 | 7 | 119 | 63 | 52 | |||
a
Length of complete coding sequence/cds is based on Drosophila melanogaster homolog
b
Length of complete cds is based on Homo sapiens ortholog
c
Length of nested PCR amplicon, excluding primer sequences; length of cds in amplicon
d
Library not completely screened for this amplicon; false positives not counted