Table 2.
Mutation Quality Classification
| Class | Scoring criteria | No. of mutations observed by TILLING | No. of mutations confirmed by sequencing |
|---|---|---|---|
| Rank 1 | Strong signal in channel 1, strong signal in channel 2 | 110 | 97 (88%) |
| Rank 2 | Strong signal in channel 1, weak signal in channel 2 | 74 | 50 (68%) |
| Rank 3 | Strong signal in channel 1, no signal channel 2 | 66 | 40 (61%) |
| Rank 4 | Weak signal in channel 1, weak signal in channel 2 | 40 | 16 (40%) |
| Rank 5 | Weak signal in channel 1, no signal channel 2 | 47 | 11 (23%) |
| Rank 6a | Aberrant | 92 | 35 (38%) |
| Totalb | 435 | 255 (59%) |
Gel files as shown in Fig. 2 were inspected manually and a rank was assigned to each of the potential mutations as observed by TILLING. Potential mutations were confirmed by resequencing.
a
Lanes in gel that look different than normal, contain multiple signals, have a signal at the same height as a background signal, etc
b
Including six unclassified mutations from gene6.