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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: J Neurosci Res. 2011 Aug 8;89(11):1773–1782. doi: 10.1002/jnr.22720

Fig. 2.

Fig. 2

Immunoblot and densitometric analysis of P-gp in primary cultures of HFAs after exposure to either CCR5-tropic HIV-1 ADA or CCR5/CXCR4 dual-tropic HIV-1 89.6 viral isolates (A), 1.0 nM gp120 (B), or IL-6 (0.5 or 10 ng/ml; C). Whole-cell lysates (50 μg) from primary cultures of HFAs were resolved on a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane. MDR1-over-expressing cell lines (MDCK-MDR1 and MDA-MDR1) were used as positive control. P-gp was detected using the monoclonal antibody C219 (1:500 or 1:300 dilution), and actin was detected using AC40 antibody (1:500 dilution). Results are expressed as mean ± SD of three separate experiments. Asterisks represent data points that are significantly different from control (*P < 0.05, ***P < 0.001).