Skip to main content
. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: J Neurosci Res. 2011 Aug 8;89(11):1773–1782. doi: 10.1002/jnr.22720

Fig. 5.

Fig. 5

Immunoblot and densitometric analysis of P-gp in primary cultures of HFAs treated with 1.0 nM gp120 (left) or IL-6 (0.5 or 10 ng/ml; right) in the presence of the NF-κB inhibitory peptide SN50 (1.0 μM). Whole-cell lysates (50 μg) from primary cultures of HFAs were resolved on a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane. An MDR1-overexpressing cell line (MDCK-MDR1) was used as positive control. P-gp was detected using the monoclonal antibody C219 (1:500 dilution), and actin was detected using AC40 antibody (1:500 dilution). Results are expressed as mean ± SD of three separate experiments. Asterisks represent data points that are significantly different from control (*P < 0.05).