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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: Biotechnol Adv. 2011 Oct 8;30(5):1185–1194. doi: 10.1016/j.biotechadv.2011.09.016

Figure 3.

Figure 3

Historical trends of cell-free protein synthesis systems. Blue squares = E. coli extract (ECE), red circles = wheat germ extract (WGE), purple triangles = insect cell extract (ICE), and green diamonds = rabbit reticulocyte lysate (RRL). (A) Cell-free protein synthesis yields for a batch reaction. (B) “Effective” cell-free protein synthesis yields for a fed-batch or continuous exchange cell-free (CECF) reaction based on the total volume of reaction and feeding solutions. (C) Reaction length for active protein synthesis in a batch cell-free reaction. (D) Rate of protein synthesis during a cell-free reaction. (E) Protein yield per dollar of NXPs (e.g., ATP, ADP, AMP, GTP, etc.) and energy source, which are the dominant substrate costs of CFPS reactions. (F) Scale of cell-free reaction volumes. Cited references can be found in Supplementary Table 1.