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Timeline: Synthetic biology milestones in the production of complex therapeutic proteins. Abbreviations: scFv: single-chain antibody variable fragment, vtPA: variant of human tissue-type plasminogen activator, GM-CSF: granulocyte macrophage colony stimulating factor, IGF-I: insulin-like growth factor I, cIFN-α: human consensus interferon-alpha, rhGM-CSF: human granulocyte macrophage colony-stimulating factor. Cited references can be found in Supplementary Table 2.
