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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods Mol Biol. 2014;1126:179–192. doi: 10.1007/978-1-62703-980-2_14

Figure 3.

Figure 3

A. Image of amylose column attached to a syringe for washing. B. Denaturing PAGE analysis of RNA from in vitro splicing and affinity purification of C complex spliceosome. Lanes from left to right are the pre-mRNA standard used for quantification (S), time points taken during the splicing reaction (0 and 60 minutes) and after RNase H digestion (80 minutes), size exclusion peak fraction loaded onto amylose column (load), elution fractions from amylose column (elution). RNA species schematized on the left are, from top to bottom, lariat intermediate, pre-mRNA, 3′ RNase H digestion product, 5′ exon, and 5′ RNase H digestion product.