Figure 3.

Anti–outer surface protein A (OspA) antibody (Ab) distribution between plots with and plots without deployment of reservoir-targeted vaccine (RTV). Blood specimens from trapped mice were tested for the presence of OspA-specific Ab by enzyme-linked immunosorbent assay. A, Mice were trapped in RTV-deployed plots NY1 and NY2 and untreated control plots during 2008–2011. B, Mice were trapped in RTV-deployed plots NY3 and NY4 and untreated control plots during 2009–2011. Vaccination resulted in a statistically significant increase in the anti-OspA Ab response after control for natural variation among plots and years, using a generalized linear mixed model (P = .002). Multiple pairwise comparisons of means by use of the Tukey honest significant difference post-hoc test revealed that differences in anti-OspA–specific Ab levels between mice captured in the vaccine plots and those captured in the control plots were statistically significant for all plots except NY3. Statistical analysis of the protective seroprevalence data was done using the χ² test. 2010 data were not included for NY3 because both values were 0.