Figure 2.

Titers of anti-Sm-p80 antibodies in immunized baboons. Enzyme-linked immunosorbent assays were performed with sera obtained from each baboon (every 4 weeks) in their respective control and experimental groups. The peak antibody titers for total immunoglobulin G (IgG), IgG1, IgG2, IgG3, IgG4, immunoglobulin M (IgM), and immunoglobulin A (IgA) in control and vaccinated groups are shown for each of the 3 vaccination strategies: recombinant protein vaccination using rSm-p80 plus glucopyranosyl lipid adjuvant (GLA)–SE (A), DNA prime boost protein with Sm-p80–VR1020 and rSm-p80 in alum (B), and similar prime and boost approach using Sm-p80–VR1020 and rSm-p80 with CpG ODNs (C). The values represent the mean (±SD) of 3 experiments.