Quantification of intracellular interferon-γ (IFN-γ), interleukin 4 (IL-4), and interleukin 17 (IL-17) secretory CD3+CD4+ T cells from rSm-p80–stimulated peripheral blood mononuclear cells. Initial gating was performed using the CD4 marker. A, The percentage of IFN-γ, IL-4, and IL-17 secretory CD3+CD4+ T cells in recipients of glucopyranosyl lipid adjuvant (GLA)–SE and rSm-p80. B, The percentage of IFN-γ, IL-4, and IL-17 secretory cells in recipients of prime boost with Sm-p80-VR1020 and rSm-p80 in alum. C, The percentage of IFN-γ, IL-4, and IL-17 secretory cells in recipients of the prime boost using Sm-p80-VR1020 and rSm-p80 with CpG ODNs groups.