Table 1.
Immunization Protocol Used to Determine the Therapeutic Efficacy of Sm-p80 Using Recombinant Protein and Prime Boost Approaches for Schistosoma mansoni in Baboons
| Group | First Infection (Week 0), Inoculum |
First Immunization, Formulation (Time) | Second Immunization, Formulation (Time) | Second Infection, Inoculum (Time) | Third Immunization, Formulation (Time) | Necropsy Time |
|---|---|---|---|---|---|---|
| GLA-SE control | 1000 S. mansoni cercariae | GLA-SE (50 µg)(week 16) | GLA-SE (50 µg) (week 20) | … | GLA-SE (50 µg) (week 24) | Week 28 |
| rSm-p80 + GLA-SE | 1000 S. mansoni cercariae | rSm-p80 (250 µg) + GLA-SE (50 µg) (week 16) | rSm-p80 (250 µg) + GLA-SE (50 µg) (week 20) | … | rSm-p80 (250 µg) + GLA-SE (50 µg) (week 24) | Week 28 |
| VR1020 + alum control | 1000 S. mansoni cercariae | VR1020 (500 µg) (week 7) | Alum (1250 µg) (week 11) | … | Alum (1250 µg) (week 15) | Week 22 |
| Sm-p80–VR1020 + rSm-p80 + alum | 1000 S. mansoni cercariae | Sm-p80–VR1020 (500 µg) (week 7) | rSm-p80 (250 µg) +alum (1250 µg) (week 11) | … | rSm-p80 (250 µg) + alum (1250 µg) (week 15) | Week 22 |
| VR1020 + CpG-ODN control | Prevailing Trichuris infection + 1000 S. mansoni cercariae | VR1020 (500 µg) (week 21) | Control CpG-ODN (250 µg) (week 25) | 1000 S. mansoni cercariae and Trichuris (week 27) | Control CpG-ODN (250 µg) (week 31) | Week 38 |
| Sm-p80-VR1020 + rSm-p80 + CpG-ODN10104 | Prevailing Trichuris infection + 1000 S. mansoni cercariae | Sm-p80–VR1020 (500 µg) (week 21) | rSm-p80 (250 µg) + CpG-ODN-10104 (250 µg) (week 25) | 1000 S. mansoni cercariae and Trichuris (week 27) | rSm-p80 (250 µg) + CpG-ODN-10104 (250 µg) (week 31) | Week 38 |
Abbreviation: GLA, glucopyranosyl lipid adjuvant.