Table 2.

SUCEST EST Summary

Total sequences	291,689
cDNA clones sequenced (5′ or 3′)	260,352
5′-end sequences	259,325
3′-end sequences	32,364
Total high-quality sequences	237,954
Success index (%)	81.6
Average insert size (bp)	1250
Average sequence size (bp)	864
Bases with phred quality ≥20/read	399

Numbers of sequenced cDNA clones and generated ESTs from 26 cDNA libraries. A total of 260,352 cDNA clones were sequenced (259,325 5′-end and 32,364 3′-end sequences). After trimming and the removal of low-quality sequences, rRNA, and bacterial sequences. 237,954 high-quality sequences with a minimum of 140 bases remained. The average insert size was evaluated by polymerase chain reaction (PCR) analysis of 400 clones from each cDNA library. The success index represents the percentage of high-quality sequences relative to the total number of sequences.