Table 3.

Sequence Assembly Results

Total sequences analyzed	237,954
Number of contigs	26,803
Number of singletons	16,338
Number of sugarcane assembled sequences (SAS)	43,141
Number of assembled sequences matching known genes	27,833
Number of clones with full-length inserts	14,409

High-quality ESTs were assembled using CAP3. A BLASTX match cutoff of ≤10^—5 was used to assign similarity. Clones were considered as having a putative full-length insert when their sequences started within the first 15 amino acids of their best BLASTX match with an E = value ≤10^—40.