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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: Methods Cell Biol. 2009 Dec 4;93:111–119. doi: 10.1016/S0091-679X(08)93006-5

Fig. 1.

Fig. 1

The vector designed for tagging Chlamydomonas genes with GFP, pHK85, has two copies of the GFP gene separated by two tandem linker sequences. The first or second GFP can be replaced by a cDNA or genomic sequence encoding a protein to be tagged with GFP at either its C- or N-terminus, respectively. Transcription is driven by the PsaD promoter and is terminated by the PsaD 3′ genomic sequence. The plasmid also encodes aphVIII as a selectable marker in Chlamydomonas.