Skip to main content
. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: J Immunol. 2013 Feb 11;190(6):2567–2574. doi: 10.4049/jimmunol.1200856

FIGURE 3.

FIGURE 3

(A) PSA preferentially cleaves iC3b. Purified human C3, C3b, and iC3b were incubated with enzymatically active PSA in the presence of 10 μM aprotinin. PSA inhibitor (1 μM) was added to control reactions. Coomassie staining of a gel run under reducing conditions revealed a cleavage product at ~37 kDa that was generated in the absence of PSA inhibitor. (B) Factor H does not have cofactor activity for PSA-mediated cleavage of C3b. Purified human C3b was incubated with enzymatically active PSA and an increasing amount of factor H. Proteins were separated by SDS-PAGE and transferred to PVDF membrane before staining with Coomassie blue.