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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: J Immunol. 2013 Feb 11;190(6):2567–2574. doi: 10.4049/jimmunol.1200856

FIGURE 5.

FIGURE 5

PSA-mediated cleavage of iC3b does not affect CR3-dependent phagocytosis. Sheep erythrocytes opsonized with iC3b were treated with PSA (100 μg/ml) or an equal amount of BSA overnight. RAW 264.7 cells were stimulated with 125 nM PMA for 10 min, after which erythrocytes were added (~20:1). The erythrocytes were phagocytised for 75 min. Erythrocytes bound but not internalized were lysed, and the number of erythrocytes phagocytosed were quantified by the colorimetric conversion of 2,7-diaminofluorene to fluorene blue (OD620) by the pseudoperoxidase activity of hemoglobin.