Skip to main content
. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: J Immunol. 2013 Feb 11;190(6):2567–2574. doi: 10.4049/jimmunol.1200856

FIGURE 6.

FIGURE 6

(A) PSA does not cleave C4. (B) PSA cleaves the C5 α-chain, leaving the β-chain intact. Purified human C4 and C5 was incubated with enzymatically active PSA in the presence of 10 μM aprotinin. Coomassie staining of a gel run under reducing conditions revealed proteolysis of the C5 α-chain. (C) PSA-mediated cleavage of C5 is inhibitory. C5-depleted normal human serum was supplemented with C5 that had been incubated with PSA or BSA overnight. This serum was added to EA. Complement activity was quantified by absorbance of the supernatant at 415 nm after hemolysis.