Table 1.
Gene Diversity and Discovery Analyzed Using ICAtools (Displaying the Number of Singletons, Paired Sequences, Trios, and Those Present in Clusters of Four or More), and WU-BLAST
| Ovary | Fin | Heart | Intestine | Skin | Muscle | Whole body | Spleen | Gill | Gut | Gonad | Brain | Eye | Liver | Kidney | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Icatools | |||||||||||||||
| 1 | 53.6 | 28.7 | 47.7 | 48.6 | 46.6 | 20.4 | 26.7 | 48.7 | 47.7 | 38.6 | 34.9 | 66.5 | 57.8 | 28.9 | 46.9 |
| 2 | 8.3 | 6.0 | 5.8 | 8.9 | 9.7 | 4.8 | 6.9 | 5.4 | 8.9 | 8.3 | 6.8 | 6.9 | 10.3 | 8.3 | 9.4 |
| 3 | 2.8 | 2.5 | 2.0 | 3.0 | 2.9 | 1.3 | 3.4 | 3.2 | 4.3 | 2.9 | 3.0 | 2.1 | 2.0 | 3.4 | 3.4 |
| 4+ | 35.3 | 62.8 | 44.5 | 39.5 | 41.8 | 73.5 | 63.0 | 42.7 | 39.1 | 50.2 | 55.3 | 24.5 | 29.9 | 59.4 | 40.3 |
| Discovery | 0.33 | 0.14 | 0.31 | 0.26 | 0.25 | 0.10 | 0.13 | 0.28 | 0.27 | 0.21 | 0.18 | 0.35 | 0.26 | 0.17 | 0.23 |
| Diversity | 0.71 | 0.45 | 0.66 | 0.70 | 0.66 | 0.34 | 0.51 | 0.65 | 0.67 | 0.58 | 0.50 | 0.76 | 0.75 | 0.48 | 0.65 |
Diversity is defined as the number of different “genes” each library contributed, divided by the library size. Discovery is defined as the number of singletons in each library divided by library size.