Figure 3.

Energetics and activity. (A) Metabolic rate was significantly reduced in females only by rapamycin feeding during the light (inactive) phase of 24-h light:dark cycle and when mice were resting; however, there was no effect during the dark (active) phase. Note that metabolic rate increased with age irrespective of treatment, and females always exhibited higher metabolic rate than males. (B) Total activity showed no clear pattern with age and was not affected by rapamycin feeding. (C) Sleep was increasingly fragmented with age (greater number of sleep bouts per hour of sleep), but rapamycin-fed males had less fragmented sleep than controls.